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. 2021 May 12;67(4):241–250. doi: 10.1262/jrd.2020-108

Fig. 5.

Fig. 5.

Suppression of MT-enhanced hyperactivation by PKA inhibitors. Percentages of motility (A) and hyperactivation (B) were determined after sperm were cultured for 4 h with 10 pM MT and various concentrations of H-89. Data represent the mean ± standard deviation (SD). (A and B) (Vehicle) medium with 0.1% (v/v) ethanol and 0.1% (v/v) dimethyl sulfoxide as vehicle; (MT) medium with 10 pM MT and vehicle; (MT + 1 µM H-89) medium with 10 pM MT, 1 µM H-89, and vehicle; (MT + 10 µM H-89) medium with 10 pM MT, 10 µM H-89, and vehicle; (MT + 100 µM H-89) medium with 10 pM MT, 100 µM H-89, and vehicle. * indicates significant differences compared with “Vehicle,” “Inhibitors,” and “MT + Inhibitors” (P < 0.05). ** indicates significant differences compared with “Vehicle,” “MT,” “MT + 1 µM H-89,” and “MT + 10 µM H-89” (P < 0.05). $ indicates significant differences compared with “Vehicle,” “MT + 1 µM H-89,” and “MT + 10 µM H-89” (P < 0.05). MT, 5-methoxytryptamine; PKA, protein kinase A.