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. 2021 Mar 5;70(10):2851–2865. doi: 10.1007/s00262-021-02895-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — CD19 and CD46 expression in lymphoma cell lines and viability post LOAd infection. Human lymphoma cell lines (BC-3, Karpas422, Daudi, DG-75 and U-698) were analyzed for CD19 and CD46 expression by flow cytometry. Filled gray histograms display isotype control staining and overlaid black lines show CD19 (a) and CD46 expression (b). Lymphoma cells were infected with LOAd viruses or replication-deficient Ad5/35 Mock virus at different virus to cell ratios (10, 50, 100 and 200 MOI). Cell viability was analyzed with MTS viability assay 96 h post infection (c) and over an extended time period (72–144 h) for cells infected with 200 MOI (d). Viability is shown as percentage viability of uninfected control cells (mean ± SD of technical quadruplicates; representative of two independent experiments). Statistical differences compared to uninfected control cells were analyzed at 200 MOI (c) or 144 h (d) with one-way ANOVA followed by Dunnett’s multiple comparisons test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)