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. 2021 Sep 7;12:5321. doi: 10.1038/s41467-021-25684-3

Fig. 3. CARM1 and XBP1s are mutually dependent for their optimal recruitment to CARM1/XBP1s target genes.

Fig. 3

a Representative cut-and-run tracks of CARM1 and XBP1s on the indicated CARM1/XBP1s target HSPA5 and DNAJB9 gene loci in cells treated with vehicle control or tunicamycin (5 μg/ml, 8 h). b ChIP-qPCR analysis for the association of XBP1s, CARM1, and H3R17me2a, an enzymatic product of CARM1, with the promoters of HSPA5 and DNAJB9 genes in control or CARM1 knockout A1847 cells treated with vehicle control or tunicamycin (5 μg/ml, 8 h). c ChIP-qPCR analysis for the association of XBP1s, CARM1, and H3R17me2a with the promoters of HSPA5 and DNAJB9 genes in control or XBP1 knockdown A1847 cells treated with vehicle control or tunicamycin (5 μg/ml, 8 h). d Sequential ChIP-qPCR analysis using an anti-CARM1 antibody (first ChIP) followed by an anti-XBP1 antibody (re-ChIP) for the promoters of HSPA5 and DNAJB9 genes in A1847 cells. Data represent mean ± SEM, n = 3 biologically independent experiments. P values were calculated using two-tailed t test.