Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 7;12:5127. doi: 10.1038/s41467-021-25348-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Cat breeds selected for Spotted (Egyptian Mau) or for Ticked (Abyssinian and Burmese) color patterns, along with their inferred Ticked genotypes. Images from Helmi Flick, with permission. b Ticked genetic interval delineated by linkage¹⁹ or shared haplotypes among cats with Dkk4 mutations. Coordinates are based on the felCat9 assembly. c Dkk4 coding mutations (red) at the signal peptide cleavage site (p.Ala18Val) or a cysteine residue (p.Cys63Tyr) involved in disulfide bridge and cysteine knot formation within cysteine-rich domain 1 (CRD1). d Representative Western blot and quantitation of variant Dkk4 proteins produced by HEK293 cells 48 h after transfection. Dkk4 proteins are detected with an anti-FLAG antibody; co-transfection with a GFP-expressing plasmid and subsequent detection of GFP provides a control for transfection efficiency. Relative intensity on the y-axis refers to the ratio of the Dkk4 band to GFP band intensity, normalized to non-mutant Dkk4; thus non-mutant Dkk4 relative intensity = 1.0 for both media and cell lysate. The experiment was repeated three times (mean +/− sem, one-way ANOVA, F = 515.2, P = 1.94 × 10⁻⁷ for media).