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. 2021 Sep 7;4:1044. doi: 10.1038/s42003-021-02577-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Stage shift is only used to locate the central hole in yellow, and image shifts are applied for data taking from other 24 holes in this example. b Multiple images stacks, 4 in this example, are recorded from different areas shown in small red circles in one hole. Pre-check of the surrounding holes is unsuitable for fast collection of images stacks from each hole.