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. 2021 Sep 7;4:1044. doi: 10.1038/s42003-021-02577-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Detection of crystals in defocused diffraction mode by yoneoLocrWatch.py in xtal mode. A hexagonal ice crystal was identified in b. The beam size is ~5 μm in a and b. c, d, e Evaluation of diffraction patterns by the same python program in diff mode. A diffraction pattern from an ice crystal was identified in e. f Detection of crystals taken in a low-magnification (300×) image in lowmagxtal mode. Crystals at the square edge were also identified, and crystals were not selected from neighboring squares. g. Registration of crystal positions to a queue list in SerialEM by FindXtalPosyoneo and cnvxtalpos2Nav.py. The crystals at the edge were automatically excluded in this case. The square size is 85 × 85 μm².