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. 2021 Sep 7;7:235. doi: 10.1038/s41420-021-00610-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A The morphology of EPCs was observed under the inverted microscope (bar = 100 μm). B The markers of EPCs were detected using immunofluorescence (CD133 presented red and the nucleus presented blue after Hoechst3342 staining; CD34 presented green and the nucleus presented blue after Hoechst3342 staining; KDR presented red and the nucleus presented blue after Hoechst3342 staining; vWF presented green and the nucleus presented blue after Hoechst3342 staining) (bar = 50 μm). C TEM showed the particle diameter of EPC-EVs (bar = 200 nm). D NTA showed the diameter distribution and concentration of EVs. E The EV marker proteins Alix, CD81, CD9, and endoplasmic reticulum protein calnexin were detected using western blot. F PKH67-labeled EPC-EVs into ECs were observed under the immunofluorescent microscope (PKH67-labeled EPC-EVs presented green and the nucleus presented blue after Hoechst3342 staining) (scale bar = 50 μm). Each experiment was repeated three times independently.