Skip to main content
. 2021 Sep 7;7:235. doi: 10.1038/s41420-021-00610-0

Fig. 2. EPC-EVs carried miR-199a-3p into ECs.

Fig. 2

A The relationship between miR-199a-3p expression in ApoE-/- mouse aorta and the area of aortic plaque. B The relationship between miR-199a-3p expression in ApoE-/- mouse serum and the serum content of pro-inflammatory factor IL-6. C miR-199a-3p expression in ECs before and after ox-LDL treatment was detected using qRT-PCR. D After EVs were treated with RNase A and detergent, miR-199a-3p expression was detected using qRT-PCR. E miR-199a-3p expression in EPC medium (EPC-CM) and EVs (EPC-EVs/-del) was detected using qRT-PCR. F EPC-EVs labeled with fluorescent Cy3-miR-199a-3p into endothelial cells were observed under the confocal laser microscope (Cy3-miR-199a-3p-labeled EVs presented red and the nucleus presented blue after DAPI staining; phalloidin-labeled endothelial cells presented green) (scale bar = 50 μm). G After ECs were co-cultured with EPC-EVs, miR-199a-3p expression in MAECs was detected using qRT-PCR. Each experiment was repeated three times independently. Measurement data are depicted as mean ± SD. The t test was used for the comparisons between two groups. One-way ANOVA was employed for the comparisons among multiple groups, followed by Tukey’s multiple comparisons test. *p < 0.05.