Fig. 5. EV-miR-199a-3p targeted SP1 and thereby inhibited ferroptosis of ECs.

A SP1 expression in each group was detected using western blot. B GSH consumption, lipid ROS production, and lipid peroxidation were detected using the kits. C The relative content of iron in MAECs was determined using the commercial kit. D, E SLC7A11 and GPX4 mRNA expression and protein levels in MAECs were detected. F Cell death rate was detected by trypan blue staining. Each experiment was repeated three times independently. Measurement data are depicted as mean ± SD. The t test was used for the comparisons between two groups. One-way ANOVA was employed for the comparisons among multiple groups, followed by Tukey’s multiple comparisons test. *p < 0.05.