Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 24;37(9):1271–1288. doi: 10.1007/s12264-021-00732-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Pathological hallmarks of PD in the SN region of PINK1 and DJ-1 gene-edited monkey brain. A, B TH immunostaining images of the AAV9-SaCas9-SgControl SN (A) and the AAV9-SaCas9-SgPINK1+DJ-1 SN (B) of monkey Old 1 at low resolution (4×). The image in B appears pale and weak with fewer surviving neurons compared with A. C, D Moderate resolution (20×) images of boxed areas in A and B. Typical dopaminergic neurons are evident in C, and many deformed dopaminergic neurons, with weakly-stained somata and almost completely lost neurites are shown in D. E, F High resolution (40×) images of the boxed areas in C and D. A typical dopaminergic neuron with intact soma (solid blue arrows) and branched processes (hollow blue arrows) can be seen in E, and a very weakly-stained dopaminergic neuron with incomplete soma boundary (solid blue arrow) and a residual process (hollow blue arrow) is shown in F. G Over 61% of nigral dopaminergic neurons are lost in AAV9-SaCas9-SgPINK1+DJ-1 on the edited side compared with the AAV9-SaCas9-SgControl side (an average of monkeys Old 1 and Middle age 1). H, I PSer129αS immunostaining images under high resolution (40×) of the AAV9-SaCas9-SgControl SN (H) and the AAV9-SaCas9-SgPINK1+DJ-1 SN (I) from monkey Old 1. Clear PSer129αS aggregates can be seen in I. J, K Enlarged images of the defined areas in H and I showing stained nuclei in H and a typical darkly-stained PSer129αS aggregate in I (arrow). L Typical example of a PSer129αS aggregate (solid arrow) and a PSer129αS deposit in a neuronal process (hollow arrow) in the AAV9-SaCas9-SgPINK1+DJ-1 SN of monkey Old 1. M Numbers of PSer129αS aggregates in the AAV9-SaCas9-SgPINK1+DJ-1 and the AAV9-SaCas9-SgControl SNs (average of monkeys Old 1 and Middle age 1). Scale bars: hollow black, 100 μm; solid black, 10 μm. Data in G and M are the median with minimum and maximum (n = 20 sections).