Fig. 5.

CARM1 knockdown impeded CARM1 coupling at K⁺ gene to decrease dorsal horn K⁺ channel expression. (a) Diagram of the timeline of this experiment. (b) Intrathecal CARM1 siRNA (CARM1 siRNA; 100 ng; 10 μL; once daily for 4 days) into naïve rats decreased the expression levels of Kcna4 and Kcnd2 mRNA in the dorsal horn at day 4 after injection. IB, Immunoblotting. **p < 0.01 versus Naïve. ^##p < 0.01 versus MS siRNA. Each group has 5 rats. Kcna4, one-way ANOVA, F_{(2, 12)} = 21.3, p = 0.0001. Kcnd2, one-way ANOVA, F_{(2, 12)} = 17.04, p = 0.0003. (c) Intrathecal CARM1 siRNA (CARM1 siRNA; 100 ng; 10 μL; once daily for 4 days) into naïve rats decreased the expression levels of Kv1.4 and Kv4.2 proteins in the dorsal horn at day 4 after injection. IB, Immunoblotting. **p < 0.01 versus Sham 7D. ^#p < 0.05, ^##p < 0.01 versus MS siRNA. Each group has 5 rats. Kv1.4, one-way ANOVA, F_{(2, 12)} = 8.698, p = 0.0046. Kv4.2, one-way ANOVA, F_{(2, 12)} = 10.28, p = 0.0025. (d) Intrathecal CARM1 siRNA (CARM1 siRNA; 100 ng; 10 μL; once daily for 4 days) into naïve rats decreased abundances of Kcna4 and Kcnd2 promoter fragments immunoprecipitated by CARM1-specific antibodies in the dorsal horn at day 4 after injection. **p < 0.01 versus Naïve. ^#p < 0.05, ^##p < 0.01 versus MS siRNA. Each group has 5 rats. Kcna4, one-way ANOVA, F_{(2, 12)} = 23.13, p < 0.0001. Kcnd2, one-way ANOVA, F_{(2, 12)} = 7.398, p = 0.0081