Fig. 6.

SNL induces Fbxo3/CARM1 signaling-mediated epigenetic silencing H3R17me2 at K⁺ channel genes promoter in dorsal horn. (a) Diagram of the timeline of this experiment. (b) BC-1215 (100 nM, 10 μL, at 4 h after injection) reversed SNL-decreased expression levels of H3R17me2 in the ipsilateral dorsal horn. IB, Immunoblotting. **p < 0.01 versus Sham 7D. ^#p < 0.05 versus SNL 7D. ⁺p < 0.05 versus SNL 7D+Veh. Each group has 5 rats. One-way ANOVA, F_{(3, 16)} = 11.26, p = 0.0003. (c) Intrathecal CARM1 siRNA (CARM1 siRNA; 100 ng; 10 μL; once daily for 4 days) decreased the level of H3R17me2 in the ipsilateral dorsal horn at day 4 after injection. *p < 0.05 versus Naïve. ^##p < 0.01 versus MS siRNA. Each group has 5 rats. One-way ANOVA, F_{(2, 12)} = 8.306, p = 0.0054. (d) Intrathecal BC-1215 (100 nM, 10 μL, at 4 h after injection) reversed SNL-decreased the Kcna4 and Kcnd2 promoter fragments immunoprecipitated by H3R17me2-specific antibodies in the ipsilateral dorsal horn of SNL rats. **p < 0.01 versus Sham 7D. ^#p < 0.05 versus SNL 7D. ⁺p < 0.05 versus SNL 7D+Veh. Each group has 5 rats. Kcna4, one-way ANOVA, F_{(3, 16)} = 19.34, p < 0.0001. Kcnd2, one-way ANOVA, F_{(3, 16)} = 25.5, p < 0.0001. (e) Intrathecal CARM1 siRNA (CARM1 siRNA; 100 ng; 10 μL; once daily for 4 days) decreased the Kcna4 and Kcnd2 promoter fragments immunoprecipitated by H3R17me2-specific antibodies in the dorsal horn of naïve rats at day 4 after injection. *p < 0.01 versus Naïve. ^#p < 0.05, ^##p < 0.01 versus MS siRNA. Each group has 5 rats. Kcna4, one-way ANOVA, F_{(2, 12)} = 8.836, p = 0.0044. Kcnd2, one-way ANOVA, F_{(2, 12)} = 7.645, p = 0.0072