Fig. 7.

BC-1215 reverses SNL-decreased CARM1, H3R17me2, Kv1.4, Kv4.2, CARM1/H3R17me2/Kv1.4 colocalization and CARM1/H3R17me2/Kv4.2 colocalization in dorsal horn. (a) Diagram of the timeline of this experiment. (b) Immunofluorescence images analysis demonstrating intrathecal BC-1215 (100 nM, 10 μL, at 4 h after injection) reversed SNL (SNL 7D) decreased CARM1-positive (red), H3R17me2-positive (green), Kv1.4-positive (blue) and CARM1/H3R17me2/Kv1.4 triple-labeled (white) immunofluorescence ipsilateral dorsal horn at day 7 after operation. Scale bar = 50 μm. Thickness = 30 μm. (c) Immunofluorescence images analysis demonstrating intrathecal BC-1215 (100 nM, 10 μL, at 4 h after injection) reversed SNL (SNL 7D) decreased CARM1-positive (red), H3R17me2-positive (green), Kv4.2-positive (blue) and CARM1/H3R17me2/Kv4.2 triple-labeled (white) immunofluorescence ipsilateral dorsal horn at day 7 after operation. Scale bar = 50 μm. Thickness = 30 μm. (d) Representative (b) and (c) statistical analyses **p < 0.01 versus Sham 7D. ^#p < 0.05 ^##p < 0.01 versus SNL 7D. Each group has 6 rats. (b) CARM1, one-way ANOVA, F_{(2, 15)} = 54.24, p < 0.0001. (b) H3R17me3, one-way ANOVA, F_{(2, 15)} = 30.4, p < 0.0001. (b) Kv1.4, one-way ANOVA, F_{(2, 15)} = 28.9, p < 0.0001. (b) Merge, one-way ANOVA, F_{(2, 15)} = 11.56, p = 0.0009. (c) CARM1, one-way ANOVA, F_{(2, 15)} = 15.52, p = 0.0002. (c) H3R17me3, one-way ANOVA, F_{(2, 15)} = 21.63, p < 0.0001. (c) Kv4.2, one-way ANOVA, F_{(2, 15)} = 35.1, p < 0.0001. (c) Merge, one-way ANOVA, F_{(2, 15)} = 8.134, p = 0.0041