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. 2021 Jun 4;22:245–255. doi: 10.1016/j.omto.2021.05.013

Figure 5.

Figure 5

METTL3 overexpression inhibits RAGE protein level in SiHa-DDP cell

(A) A CCK-8 assay was used to measure the cisplatin sensitivity in SiHa-DDP cells. ∗∗∗p < 0.001 versus SiHa group. (B) The expression level of RAGE proteins in SiHa-DDP cells was detected by western blotting. (C) The SiHa-DDP cell line with METTL3 overexpression was observed under the bright field of a microscope and fluorescence (GFP) was observed under the dark field of a microscope (original magnification, ×100). (D and E) The expression of METTL3 and RAGE proteins in SiHa-DDP cells after METTL3 cDNA transfection was detected by western blotting (D). Quantitative results of panel D were shown (E)∗∗∗p < 0.001 versus vector group. (F) Cisplatin sensitivity in SiHa-DDP cells after METTL3 overexpression was analyzed by a CCK-8 assay. ∗p < 0.05, ∗∗p < 0.01 versus vector group. (G and H) The apoptosis rate was analyzed in SiHa-DDP cells treated with METTL3 cDNA transfection and cisplatin by flow cytometry (G). Quantitative results of panel G were shown (H). ∗∗∗p < 0.001 versus vector group; ###p < 0.001 versus MOE group; &&p < 0.01 versus vector + DDP group. (I) The expression levels of METTL3 and RAGE proteins in SiHa cells after METTL3 cDNA transfection and treatment with cisplatin were detected by western blotting. MOE, METTL3 overexpression.