ZIC2 is upregulated in NSCLC
(A) Comparison of ZIC2 expression between NSCLC tissues and the matched adjacent normal tissues (ANTs) in The Cancer Genome Atlas (TCGA). (B) Expression level of ZIC2 in 109 ANT, 514 lung adenocarcinoma (LUAD), and 496 lung squamous cell carcinoma (LUSC) tissues in TCGA. Each bar represents the mean ± SD. (C) Expression level of ZIC2 in 340 ANT, 1109 LUAD, and 414 LUSC tissues in AE-meta. Each bar represents the mean ± SD. (D) ZIC2 mRNA expression in paired NSCLC tissues compared with their matched ANT patient samples. Transcript levels were normalized to GAPDH expression. Each bar represents the mean ± SD of three independent experiments. ∗ p < 0.05. (E) ZIC2 protein expression in paired NSCLC tissues compared with their matched ANT patient samples. α-tubulin served as the loading control. (F) ZIC2 mRNA expression in normal lung epithelial cell lines and NSCLC cell lines. Transcript levels were normalized to GAPDH expression. Each bar represents the mean ± SD of three independent experiments. ∗ p < 0.05. (G) ZIC2 protein expression in normal lung epithelial cell lines and NSCLC cell lines. α-tubulin served as the loading control.