Figure 3.

Silencing ZIC2 inhibits tumorigenesis of lung cancer cells in vivo

(A) ZIC2 expression in endogenous ZIC2-knockdown A549 and NCI-H520 cell lines. Transcript levels were normalized to GAPDH expression. α-tubulin served as the loading control. Each bar represents the mean ± SD of three independent experiments. ∗ p < 0.05. (B) Metastatic lung tumor nests (upper panel, red arrow) and lung metastatic tumor tissues (lower panel) in the indicated mice group. Scale bars, 200 μm. (C) The numbers of tumor nodules in the lung section of each group. (D) Kaplan-Meier survival curves of the mice after tail-vein injection of the indicated lung cancer cells. (E) The number of CTCs in blood samples of the indicated mice group. Each bar represents the median values ± quartile values.