Figure 5.

Silencing ZIC2 abrogates anoikis resistance in lung cancer cells in vitro

(A−C) The effect of ZIC2 on proliferation of lung cancer cells was assessed by CCK-8 assay (A and B) and colony-formation assay (C). Each bar represents the mean value ± SD of three independent experiments. n.s, no significance. (D and E) The effect of ZIC2 on the activities of caspase-3 (D) and caspase-9 (E) was detected by the cleaved forms of these two proteins. Each bar represents the mean value ± SD of three independent experiments. ∗ p < 0.05. (F) The effect of ZIC2 on apoptosis of lung cancer cells was assessed under suspension growth conditions. Each bar represents the mean value ± SD of three independent experiments. ∗ p < 0.05. (G) The effect of ZIC2 on mitochondrial membrane potential of lung cancer cells under suspension growth condition. Each bar represents the mean value ± SD of three independent experiments. ∗ p < 0.05.