Table 2.

Sequences of RT-PCR primers and probes approved by CDCs in different countries.

Country	Target gene	Primer and probe	Sequence (5′-3′)	Position (Reference sequence: NC_045512.2)
China12	ORF1ab	ORF1ab-F	ccctgtgggttttacacttaa	13,342–13,362
		ORF1ab-R	acgattgtgcatcagctga	13,442–13,460
		ORF1ab-P	FAM-ccgtctgcggtatgtggaaaggttatgg-BHQ1	13,377–13,404
	N	N-F	ggggaacttctcctgctagaat	28,881–28,902
		N-R	cagacattttgctctcaagctg	28,958–28,979
		N-P	FAM-ttgctgctgcttgacagatt-TAMRA	28,934–28,953
USA13	N	N1-F	gaccccaaaatcagcgaaat	28,287–28,306
		N1-R	tctggttactgccagttgaatctg	28,335–28,358
		N1-P	FAM-accccgcattacgtttggtggacc-BHQ1	28,309–28,332
	N	N2-F	ttacaaacattggccgcaaa	29,164–29,183
		N2-R	gcgcgacattccgaagaa	29,213–29,230
		N2-P	FAM-acaattttgcccccagcgcttcag-BHQ1	29,188–29,210
	N	N3-F	gggagccttgaatacaccaaaa	28,681–28,702
		N3-R	tgtagcacgattgcagcattg	28,732–28,752
		N3-P	FAM-aycacattggcacccgcaatcctg-BHQ1	28,706–28,727
Japan14	N	N-F	aaattttggggaccaggaac	29,125–29,144
		N-R	tggcagctgtgtaggtcaac	29,280–29,299
		N-P	FAM-atgtcgcgcattggcatgga-BHQ	29,222–29,241
Germany11	RdRP	RdRp-F	gtgaratggtcatgtgtggcgg	15,431–15,452
		RdRP-R	caratgttaaasacactattagcata	15,505–15,530
		RdRp-P	FAM-caggtggaacctcatcaggagatgc-BBQ	15,470–15,494
	E	E-F	acaggtacgttaatagttaatagcgt	26,269–26,294
		E-R	atattgcagcagtacgcacaca	26,360–26,381
		E-P	FAM-acactagccatccttactgcgcttcg-BBQ	26,332–26,357

E envelope protein, F forward primer, N nucleocapsid protein, ORF open reading frame, P probe, R reverse primer, RdRp RNA-dependent RNA polymerase.