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Comparison of CRISPR-based and RT-PCR assays.
| Parameters or aspects | CRISPR-based assays | RT-PCR assays |
|---|---|---|
| Target gene | E and N | ORF1ab, N, E, and RdRP |
| TAT | 30–40 min | 4 h |
| Assay results | Qualitative | Quantitative |
| Specific instrument required | No | Yes |
| FDA or EUA approval | Not yet | Yes |
E envelope protein, N nucleocapsid protein, EUA European emergency use authorization, FDA US Food and Drug Administration, ORF open reading frame, RdRp RNA-dependent RNA polymerase, TAT turn-around time.
