Table 1.
Cycle threshold (Ct) values of the RT-qPCRs targeted on glycoprotein (GP) and polymerase genes, and results of the conventional RT-PCR targeted on the GP 1 kb.
| Sample ID | Ct (GP) | GP 1 kb | Ct (polymerase) |
|---|---|---|---|
| Positive control | 32.85 | – | 32.59 |
| Yar 4 | 25.43 | NEG | 28.34 |
| Yar 8 | 28.91 | POS (MW776596) | 26.45 |
| Yar 10 | 25.70 | NEG | 28.56 |
| Yar 11 | 36.06 | NEG | 37.79 |
| Yar 13 | 22.93 | POS (MW776597) | 24.80 |
| Yar 16 | 24.86 | NEG | 28.77 |
| Yar 26 | 21.45 | POS (MW776598) | 23.29 |
| Yar 28 | 28.66 | NEG | 30.93 |
| Yar 31 | 25.10 | POS (MW776599) | 25.25 |
| Dam 13 | 25.31 | NEG | 30.78 |
The GP 1 kb RT-PCR is less sensitive than the RT-qPCR, explaining lower positive samples. Only the lowest Ct values (high viral load) obtained for the polymerase test gave signals in the conventional RT-PCR, and provided subsequent sequencing. Accession numbers are given between parentheses.