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. 2021 Sep 3;4(11):e202101057. doi: 10.26508/lsa.202101057

Figure S2. Live cell imaging of U2OS overexpression model.

Figure S2.

(A) Localization of ARH3 protein as checked by live cell imaging for GFP-tagged ARH3 (green) with mitotracker (red) and DAPI (blue) staining in separate and merged images in ARH3(WT)-GFP, ARH3(CAT)-GFP, and ARH3(V335G)-GFP in transfected BE(2)-M17 neuroblastoma cells. Size bar indicates 10 µm. (B) Recruitment of ARH3-GFP to the nucleus and DNA damage sites (white arrows) upon laser-induced DNA damage. The ARH3(V335G)-GFP protein does not relocate upon laser-induced DNA damage. (C) Expression of ARH3(WT)-GFP and ARH3(V335)-GFP protein in transfected U2OS cells. Size bar indicates 10 µm.