Fig. 1.

Correlation of metabolite levels in mouse plasma and in artificial plasma matrix samples. (A-D) Calibration curves for (A) carnitine, (B) choline, (C) betaine and (D) trimethylamine-N-oxide (TMAO) in artificial plasma matrix. Carnitine, choline, betaine and TMAO were spiked into mouse plasma at three concentrations (2,500, 5,000 and 10,000 ng/mL) per analyte. The concentrations determined in metabolite-spiked mouse plasma samples correlate with calibration curves prepared in artificial matrix. All concentrations determined for metabolite-spiked mouse plasma samples were in the range of 80%–120%. No statistically significant difference was detected between metabolite-spiked mouse plasma samples and the respective concentrations in artificial matrix using an ordinary one-way ANOVA with Sidak's multiple comparison test.