. 2021 May 20;11(4):398–404. doi: 10.1016/j.jpha.2021.05.001

Table 2.

Comparison of immunological and chemical methods for the determination of aflatoxins.

Item	Pretreatment	Sensitivity	Type	Percent recovery (%)				Equipment	Speed	Cost (RMB/test)	Personnel	Refs.
Item	Pretreatment	Sensitivity	Type	Hordei Fructus Germinatus	Ziziphi Spinosae Semen	Persicae Semen	Coicis Semen	Equipment	Speed	Cost (RMB/test)	Personnel	Refs.
ELISA	Direct dilution or extraction, 20–60 min	ng/mL	AFB₁	82.8–95.9	74.7–88.5	94.1–101.9	84.0–89.1	Fluorophotometer (10,000–30,000 RMB)	60 min/test	30–50	Ordinary personnel can operate	[58]
ELISA	Direct dilution or extraction, 20–60 min	ng/mL	AFTs	98.4–110.4	87.0–98.6	104.0–112.0	86.8–103.3	Fluorophotometer (10,000–30,000 RMB)	60 min/test	30–50	Ordinary personnel can operate	[58]
HPLC	Immunoaffinity column, 4–8 h	ng/mL	AFB₁	61.3–70.9	57.0–61.8	68.1–78.5	63.9–69.7	HPLC (<100,000 RMB)	3 h/test	200–300	Requires trained personnel to operate	[58]
HPLC	Immunoaffinity column, 4–8 h	ng/mL	AFTs	62.5–78.5	58.1–67.8	69.2–84.0	65.1–74.5	HPLC (<100,000 RMB)	3 h/test	200–300	Requires trained personnel to operate	[58]

AFB1: aflatoxin B1; AFTs: total aflatoxin.