Table 2.
Method validation.
| Compound | Calibration curve | r | LOD (ng/mL) | LOQ (ng/mL) | Precision (RSD, %) |
Repeatability (RSD, %) | Stability (RSD, %) | Recovery |
||
|---|---|---|---|---|---|---|---|---|---|---|
| Intra-day | Inter-day | Mean (%) | RSD (%) | |||||||
| Gallic acid | y = 1000000x+47943 | 0.9996 | 4.1 | 10.0 | 0.45 | 2.12 | 1.70 | 2.80 | 103.02 | 3.64 |
| Methyl gallate | y = 1000000x+18437 | 0.9997 | 0.5 | 2.3 | 0.92 | 2.98 | 5.61 | 2.22 | 115.05 | 4.07 |
| Luteolin | y = 2000000x+30415 | 0.9998 | 0.1 | 0.5 | 1.70 | 6.92 | 6.22 | 2.94 | 106.82 | 6.16 |
| Quercetin | y = 528108x-957957 | 1.0000.0000 | 2.9 | 4.8 | 1.80 | 6.07 | 2.11 | 4.88 | 90.29 | 4.66 |
| Myricetin | y = 651452x-7138 | 0.9998 | 2.5 | 4.2 | 3.01 | 7.72 | 3.03 | 4.43 | 102.17 | 6.82 |
| ECG | y = 494967x+20522052 | 1.0000.0000 | 5.3 | 22.1 | 1.38 | 5.08 | 0.90 | 3.69 | 107.22 | 4.94 |
| Astragalin | y = 837204x+65286 | 0.9996 | 0.2 | 0.4 | 1.27 | 4.46 | 3.20 | 3.57 | 110.32 | 4.69 |
| Quercitrin | y = 746261x+3612 | 0.9999 | 0.5 | 2.5 | 1.78 | 3.21 | 3.31 | 3.67 | 100.65 | 3.42 |
| EGCG | y = 585905x+410 | 1.0000.0000 | 2.5 | 4.2 | 0.76 | 3.21 | 1.07 | 2.79 | 96.47 | 3.61 |
| Myricetrin | y = 1000000x+11877 | 0.9998 | 0.5 | 2.2 | 1.25 | 2.80 | 1.41 | 3.34 | 101.92 | 2.23 |