Table 3.

Stability investigation of the three saikosaponins and saikogenins in HBSS, and the three saikogenins in FaSSIF (n = 3).

Matrix	Analyte	Conc. (nM)	Autosampler (4 °C for 8 h)		Bench-top (room temperature for 6 h)		Processed samples (−80 °C for 8 h)		Freeze-thaw (three cycles at −80 °C
			RE (%)	RSD (%)	RE (%)	RSD (%)	RE (%)	RSD (%)	RE (%)	RSD (%)
HBSS	SSa	1	−3.60	4.06	−1.06	3.34	−10.40	5.73	−7.46	2.31
		10	0.91	3.23	2.00	3.99	2.87	1.90	8.65	5.02
		100	−1.88	2.24	0.62	2.30	−1.50	2.50	1.36	4.18
	SSb2	1	0.92	4.28	0.01	4.51	3.52	6.88	2.62	9.58
		10	0.35	0.15	5.34	7.84	6.93	8.68	3.06	7.60
		100	5.11	2.79	−0.27	1.49	4.04	0.91	8.34	4.11
	SSd	1	−5.69	3.00	0.83	7.67	1.51	11.60	−1.11	3.39
		10	−0.46	1.31	8.37	10.07	−0.37	2.32	5.76	2.26
		100	−0.92	3.80	−8.09	1.93	−5.31	3.18	12.04	0.32
	SGF	10	−6.82	1.63	−2.96	2.40	5.21	7.10	−0.51	2.53
		100	−7.36	2.93	−7.96	6.86	3.65	3.42	−3.53	8.97
		1000	−6.57	0.74	−2.73	2.39	−5.44	2.54	0.47	2.78
	SGD	8	−1.59	5.06	4.25	6.79	−0.13	1.28	−2.28	6.91
		80	−0.97	3.48	−3.00	6.24	1.94	5.17	−0.85	6.80
		800	−3.88	1.25	−2.58	2.48	0.09	3.02	−0.43	1.02
	SGG	22	2.23	5.73	−1.00	2.76	3.75	6.02	−1.34	10.41
		220	0.41	1.93	−2.18	6.87	−0.01	3.59	1.41	2.64
		2200	−6.03	0.37	−6.91	1.40	−2.28	5.87	−3.22	7.33
FaSSIF	SGF	10	7.49	3.27	6.33	4.67	1.06	3.88	−0.46	8.31
		140	8.15	0.51	3.72	1.45	7.74	2.75	−3.08	1.29
		1960	3.48	2.73	6.82	3.37	−5.13	3.84	2.50	5.34
	SGD	10	5.55	4.60	4.47	4.99	−4.21	7.29	3.85	5.12
		140	−3.97	7.47	−6.46	4.19	−1.74	3.96	−2.71	2.06
		1960	−2.04	9.32	8.29	1.51	−7.05	6.90	−5.38	1.24
	SGG	25	1.89	8.55	−0.37	3.79	−2.77	6.06	3.59	10.69
		250	1.54	6.13	−1.48	7.18	−3.16	1.73	−3.46	13.21
		2500	4.74	7.93	3.68	0.93	−3.98	0.82	−10.93	4.95