Fig. 2.

Hyperoxygenation treatment increased the expression of MMP-2, MMP-9, and tPA in the hippocampus of Tg-APP/PS1 mice. (A) Real-time PCR data showing transcript levels of Bace1, Bace2, Psen1, Psen2, Adam10, and Apoe genes in the hippocampus of wildtype control (WT-CON), wildtype mice exposed to hyperoxygenation (WT-HO₂), Tg-APP/PS1 control mice (Tg-CON), and Tg-APP/PS1 mice exposed to hyperoxygenation (Tg-HO₂). n=6-7 animals/group, 4 PCR repeats. (C) Western blot data showing expression levels of APP, BACE1, PSEN1, and ApoE in the hippocampus of WT-CON, WT-HO₂, Tg-CON, and Tg-HO₂ mice. n=6~8 animals/group, 4~11 repeats. (D) Real-time PCR data showing transcript levels of Mmp-2, Mmp-9, tPA, Ide, Nep, and Lrp1 genes in the hippocampus of WT-CON, WT-HO₂, Tg-CON, and Tg-HO₂ mice. n=6~7 animals, 4 data points. (E) Western blot data showing expression levels of MMP-2, MMP-9, tPA, uPA, and Ide genes in the hippocampus of WT-CON, WT-HO₂, Tg-CON, and Tg-HO₂ mice. Tissue samples were prepared at the time point of Figure 1A. n=6~8 animals/group, 4~10 data points. Data are presented as mean±SEM. *^,**difference between indicated groups. *p<0.05; **p<0.01 (two-way ANOVA and Bonferroni post hoc test).