Fig. 4.

Hyperoxygenation increased the expression of MeCP2 and Pea3 in the hippocampus of Tg-APP/PS1 mice. (A) The proximal promoter regions of the Mmp-2, Mmp-9, and tPA genes and the locations of known transcription factor binding sites (Runx2, Peas3, NF-kB, STAT) and CpG islands. (B) Experimental design for hyperoxygenation treatment in wildtype and Tg-APP/PS1 mice. 100% O₂ at 2 ATA for 60 min daily for 28 days (HO₂). (C) Real-time PCR data showing transcript levels of Mecp2, Runx2, and Pea3 in the hippocampus of WT-CON, WT-HO₂, Tg-CON, and Tg-HO₂ mice. n=8~9 animals/group, 4~9 repeats. (D, E) Western blot images showing expression levels of MeCP2 and PEA3 proteins in the hippocampus (D) of WT-CON, WT-HO₂, Tg-CON, and Tg-HO₂ mice and their quantification levels (E). n=6~9 animals/group, 3~7 repeats. Data are presented as mean±SEM. *^,**difference between indicated groups. *p<0.05; **p<0.01 (Student’s t-test; one-way ANOVA and Newman–Keuls post hoc test; two-way ANOVA and Bonferroni post hoc test).