Fig. 3.
In vitro studies. (A, B) Quantification of mLNVs cytotoxicity in melanoma cell cultures. Cells were exposed to a wide range (from 0.5 to 5 μg/mL) of DOX-equivalent mLNVs concentrations for 24 h and 48 h. Results are expressed as cell viability %. The data represent the mean value ± SD of triplicate cultures (*** = t0.999). (C) Representative confocal microscopy images showing nuclear accumulation of DOX from free DOX and mLNVs-DOX exposure at a concentration of 2 μg/mL for 2, 4 and 6h. The columns show DOX fluorescence images (red), cell nuclei stained with Hoechst (blue) and the corresponding merged images. Magnification 10x. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)