Fig. 2. MicroRNA (miRNA)-messenger RNA (mRNA) regulatory network. Among 22 differentially expressed miRNAs (DEmiRNAs), 14 netDEmiRNA had a network of miRNAs–mRNAs that has a threshold for the minimum number of miRNA-target interactions of 4 and false discovery rate < 0.01. Bold blue boxes indicate differentially expressed mRNAs between normal and cancer bile samples. CCNT2, cyclin T2; ALDH5A1, aldehyde dehydrogenase 5 family member A1; DICER1, dicer 1, ribonuclease III; CNOT6, CCR4-NOT transcription complex subunit 6; ITGB1, integrin subunit beta 1; AKT3, AKT serine/threonine kinase 3; CTNNBIP1, catenin beta interacting protein 1; DNMT1, DNA methyltransferase 1; ZEB2, zinc finger E-box binding homeobox 2; WDR37, WD repeat domain 37; MYB, MYB proto-oncogene, transcription factor; DCBLD2, discoidin, CUB and LCCL domain containing 2; RPS6KA3, ribosomal protein S6 kinase A3; EGR1, early growth response protein 1; NLK, nemo like kinase; PLAG1, PLAG1 zinc finger; BCL2L11, BCL2 like 11; CASP8, caspase 8; BMPR2, bone morphogenetic protein receptor type 2; VHL, Von Hippel-Lindau syndrome; IRS1, insulin receptor substrate 1; CUL5, cullin 5; FOS, Fos protooncogene; PIK3CG, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma; NCOA3, nuclear receptor coactivator 3; SIRT1, sirtuin 1; PTEN, Phosphatase and tensin homolog; BCL2, B-cell lymphoma 2; VEGFA, vascular endothelial growth factor A; CDK6, cyclin dependent kinase 6; MYC, MYC proto-oncogene; MMP2, matrix metallopeptidase 2; DNMT3B, DNA methyltransferase 3 beta; LOX, lysyl oxidase; ADAM12, ADAM metallopeptidase domain 12; HMGCR, 3-hydroxy-3-methylglutaryl-CoA reductase; SERPINH1, serpin family H member 1; COL4A2, collagen type IV alpha 2 chain; FBN1, fibrillin 1; LAMC2, laminin subunit gamma 2; COL5A2, collagen type V alpha 2 chain; TDG, thymine-DNA glycosylase; SPARC, secreted protein acidic and rich in cysteine; TET2, Tet methylcytosine dioxygenase 2; IRS1, insulin receptor substrate 1; BMPR2, bone morphogenetic protein receptor type 2; PIK3R1, phosphoinositide-3-kinase regulatory subunit 1; COL4A1, collagen type IV alpha 1 chain; AKT2, AKT serine/threonine kinase 2; LRP6, LDL receptor related protein 6.