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. 2021 Jul 21;218(9):e20210324. doi: 10.1084/jem.20210324

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© 2021 Chen et al.

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Figure 7. — IEC-derived Foxo1 maintains epithelial TJs through bacterial SCFA metabolism.(A) Relative abundance of indicated bacterial genera in fecal samples from different groups. (B) Quantification of indicated SCFAs from feces of Foxo1^fl/fl and Vil1^CreFoxo1^fl/fl mice under separated (Sep) housing and cohousing (Co) conditions. (C) Representative immunofluorescence staining for mucus using occludin (red) or F-actin (green) with DAPI (blue) distal colon segments between Foxo1^fl/fl and Vil1^CreFoxo1^fl/fl mice under separated housing (left) or cohousing (right) condition. The right column represents a magnified image from the white box in the left column. Scale bar, 15 µm. (D) Quantitative analysis of occludin colocalization with F-actin in colonic IECs as in G. Occludin, occluding-positive cells, no colocalization; F-actin, F-actin single-positive cells, no colocalization; Double, occludin and F-actin double-positive cells. (E) Intestinal barrier permeability was assessed by FITC-dextran (FITC-Dex) assay between Foxo1^fl/fl and Vil1^CreFoxo1^fl/fl mice under the indicated treatment. (F) Body weights of Vil1^CreFoxo1^fl/fl mice with the indicated treatment during DSS-induced colitis. Data are representative of two independent experiments (A, C, E, and F) or are pooled from two independent experiments (B and D). *, P < 0.05 (Student’s t test; error bars represent SD).