Fig. 8.

Therapeutic effect in orthotopic GBM-bearing mice after repetitive oral administration and PTT laser irradiation. (a) Schematic illustration of the experimental strategy for repetitive oral administration and PTT laser irradiation. The GBM tissues in the brain were harvested and analyzed 7 days after repetitive treatment. (b) Temperature measurements of the head of GBM-bearing mice during PTT laser irradiation for 180 s. Data were expressed as the mean ± SEM (n = 5). ***P < 0.001 versus No treatment (PBS vehicle) group. ^##P < 0.01 versus AuNP group. ^###P < 0.001 versus AuNP group. (c) Thermal images of mice heads (dashed line) during PTT laser irradiation. (d) Nissl dye-stained images of GBM-bearing brain sections after 3 times repetitive oral administration and PTT laser irradiation. Dashed line: GBM tissue area. Magnification: × 40. (e) Proportion of GBM in the brain from the Nissl results. (f) H&E stain and TUNEL immunostain of GBM tissues. Green color: TUNEL-positive apoptotic cells. Sale bar: 50 μm. (g) Mean fluorescence intensity (MFI) of TUNEL results. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)