| Antibodies |
|
|
|
| Mouse monoclonal anti-CYP4A |
Santa Cruz |
Cat# sc-271983; RRID: AB_10715105 |
| Mouse monoclonal anti-RNF8 |
Santa Cruz |
Cat# sc-271462; RRID: AB_10648902 |
| Mouse monoclonal anti-Ub |
Santa Cruz |
Cat# sc-8017; RRID: AB_628423 |
| Mouse monoclonal HA-probe |
Santa Cruz |
Cat# sc-7392; RRID: AB_627809 |
| Mouse monoclonal β-Actin |
Santa Cruz |
Cat# sc-47778; RRID: AB_626632 |
| Rabbit polyclonal anti-EHHADH |
Proteintec |
Cat# 26570-1-AP; RRID: AB_2880556 |
| Rabbit polyclonal anti-HADHA |
Proteintec |
Cat# 10758-1-AP; RRID: AB_2115593 |
| Rabbit polyclonal anti-KRT23 |
Origene |
Cat# TA321839 |
| Rabbit monoclonal anti-RXRα |
Abcam |
Cat# ab125001; RRID: AB_10975632 |
| Rabbit polyclonal anti-Histon H3 |
Abcam |
Cat# ab1791; RRID: AB_302613 |
| Rabbit polyclonal anti-PPARα |
Abcam |
Cat# ab126285 |
| Rabbit monoclonal anti-K48-linkage Ubiquitin |
Cell Signaling Technology |
Cat# 8081; RRID: AB_10859893 |
|
| Bacterial and virus strains |
|
|
|
| AAV8-Alb-EGFP; -let-7 sponge; -pre-let-7c-1
|
This paper |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Protease inhibitor cocktail |
Sigma-Aldrich |
Cat# P9599 |
| Protein A Agarose |
Sigma-Aldrich |
Cat# P2545 |
| Wy-14,643 |
APExBIO |
Cat# A4305 |
| MG-132 |
Sigma-Aldrich |
Cat# M8699 |
| Dexamethasone |
Sigma-Aldrich |
Cat# D2915 |
| Insulin-Transferrin-Serenium |
Sigma-Aldrich |
Cat# I3146 |
| Glutamax Supplement
|
Thermo Fisher Scientific |
Cat# 35050079 |
| HEPES |
Thermo Fisher Scientific |
Cat# 15630080 |
| Humulin R (human recombinant insulin) |
Eli Lilly |
N/A |
|
| Critical commercial assays |
|
|
|
| Taqman microRNA Assay |
Thermo Fisher Scientific |
Cat# 4427975 Assay ID:000377; 000378; 000379; 002283; 002406; 000382; 002282; 002221; 000577 |
| L-Type Triglyceride M |
Fujifilm Wako Diagnostics |
Cat# 994-02891; 990-02991 |
| Cholesterol E |
Fujifilm Wako Diagnostics |
Cat# 999-02601 |
| HR Series NEFA-HR(2) |
Fujifilm Wako Diagnostics |
Cat# 999-34691; 995-34791; 991-34891; 993-35191; 276-76491 |
| SimpleChIP Plus Enzymatic Chromatin IP kit |
Cell Signaling Technology |
Cat# 9005 |
| ALT assay kit |
Catachem Inc |
Cat# V165-12 |
|
| Deposited data |
|
|
|
| Raw and analyzed data |
This paper |
GEO: GSE165521
|
|
| Experimental models: Cell lines |
|
|
|
| Human: Hep G2 cells |
ATCC |
Cat# HB-8065 |
| Mouse: Hepa-1c1c7 cells |
ATCC |
Cat# CRL-2026 |
|
| Experimental models: Organisms/strains |
|
|
|
| Mouse: C57BL/6 |
Charles River Laboratories |
Strain Code: 027 |
| Mouse: Ppara wild-type (Ppara+/+) |
Brocker et al., 2017
|
N/A |
| Mouse: Hepatocyte-specific Ppara knockout (PparaΔHep) |
Brocker et al., 2017
|
N/A |
| Mouse: Alb-Cre
|
Yakar et al., 1999
|
N/A |
| Mouse: let7b/c2 flox |
Madison et al., 2013
|
N/A |
|
| Oligonucleotides |
|
|
|
| miRIDIAN microRNA Mimic Negative Control #1 |
Dhamacon |
Cat# CN-001000-01-05 |
| miRIDIAN microRNA hsa-let-7c-5p mimic |
Dhamacon |
Cat# C-300477-03-0005 |
| let-7 sponge; see Figure S4
|
Integrated DNA Technologies |
N/A |
| Primers for qPCR; see Table S1
|
This paper |
N/A |
|
| Recombinant DNA |
|
|
|
| pscAAV-ALBp-EGFP |
Kim et al., 2019
|
N/A |
| pSR449B, pAAV8RC |
Park et al., 2009
|
N/A |
| pCMV-MIR-MirLet7c-1 |
Origene |
Cat# SC400710 |
| pSG5-mouse Rxra
|
Leid et al., 1992
|
N/A |
| pCMV6-mouse Rnf8
|
Origene |
Cat# MR207821 |
| pRK5-HA-Ubiquitin-WT |
Addgene |
Cat# 17608 |
| pGL4.27 |
Promega |
Cat# E8451 |
| phRL-TK |
Promega |
Cat# E6241 |
| pmirGLO |
Promega |
Cat# E1330 |
|
| Software and algorithms |
|
|
|
| GraphPad Prism 7.0 |
https://www.graphpad.com:443/
|
N/A |
| Ingenuity Pathway Analysis |
https://digitalinsights.qiagen.com/products-overview/discovery-insights-portfolio/content-exploration-and-databases/qiagen-ipa/
|
N/A |
