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qPCR validation of the RNA sequencing (RNAseq) of upregulated genes from the gastric fundus and corpus. A: acyl-CoA synthetase long-chain family member 5 (ACSL5); B: carbonic anhydrase 12 (CA12); C: growth hormone 1 (GH1); and D: protein kinase cGMP-dependent 2 (PRKG2). Data are presented as means ± SE. n = 24/region. *P < 0.05, **P < 0.01 ***P < 0.001.
