Figure 3.

Lactate dehydrogenase (LDH) isoform A and isoform B (Ldha and Ldhb, respectively) mRNA expression in a healthy mouse nephron. Using publicly available mouse renal single-cell RNA-sequencing data [GSE129798, Ransick et al. (30)] and the Kidney Cell Explorer tool (https://cello.shinyapps.io/kidneycellexplorer/), we investigated Ldha and Ldhb transcript expression throughout nephron segment-specific clusters. A: multiple cell populations were identified in this dataset, which is shown in the schematic along with the description of each population ID (NA indicates unidentified cells). The distinction between female (4, 6, and 8) and male (3, 5, and 7) segments (S1−S3) of proximal tubule cells is also shown. B: log-normalized expression plots illustrating the Ldha and Ldhb transcript distribution throughout the nephron. Ldha transcript expression was strongest in proximal segments and the loop of Henle, whereas Ldhb expression was present in proximal segments but was strongest in the distal tubule and collecting duct segments. C: average transcript expression in nephron segments of both isoforms. D: proportion of cells expressing the gene highlighting the differential distribution.