FIG. 6.
Effects of Ad E proteins on p73-induced G1 arrest and p73-induced endogenous p21Waf1 expression. (A) pCMV-p73 was transfected either alone or together with the indicated plasmids in the presence of GFP-tropomyosin into Saos-2 cells. Forty-eight hours after transfection, cells were collected and the cell cycle profile of GFP-positive cells was analyzed by FACScan. The effects of the adenoviral proteins on G1 increase by p73 are shown relative to the increase in G1 caused by the expression of p73 alone, which was set to 100%. The given data represent the mean values of four different experiments, and the error bars indicate the variations between the independent experiments. (B) Expression of endogenous p21Waf1 after transient transfection of Saos-2 cells by p73 alone or in the presence of the indicated plasmids. Forty-eight hours after transfection, lysates were made and the p21Waf1 expression was analyzed by Western blot analysis.