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. 2021 Jun 25;246(17):1869–1883. doi: 10.1177/15353702211023473

Figure 4.

Figure 4.

Downregulation of KDM1A or KDM3A significantly impaired tumor growth in vitro through cell cycle arrest. (a) siRNA was used to inhibit KDM1A or KDM3A expression in Panc-1 and CFPAC-1 cells and was verified by qRT–PCR (left) and Western blotting (right), n = 3. (b) The EdU assay showed that the knockdown of KDM1A significantly reduces cell proliferation in Panc-1 cells, n = 6. (c–d) The EdU assay showed that the knockdown of KDM3A significantly reduces cell proliferation in Panc-1 cells, n = 6. (e) The colony formation assay was performed to detext the ability of colony formation upon KDM1A or KDM3A knockdown. Either the knockdown of KDM1A or KDM3A reduced colony formation in Panc-1 and CFPAC-1 cells. (f–g) Cell cycle analysis was performed by flow cytometry. KDM1A siRNA led to cell arrest in G0G1 in CFPAC-1 and Panc-1 cells. (h) Knockdown of KDM3A also led to cell arrest in G0G1 in BxPC-3 and CFPAC-1. NC: negative control; Si: siRNA; ***P < 0.001. Scale bars correspond to 50 μm. (A color version of this figure is available in the online journal.)