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. 2021 May 8;246(17):1895–1906. doi: 10.1177/15353702211011576

Figure 1.

Figure 1.

Hypoxia induces exosome secretion from CRC cells to promote proliferation while inhibiting apoptosis in normoxic tumor cells in vitro. (a) Transmission electron microscopy images of exosomes isolated from conditioned medium of HCT116 cells under normoxia (N-Exos) and hypoxia (H-Exos); Scale bar, 200 nm. (b) NTA of N/H-Exos isolated by ultracentrifugation. (c) Western blot analysis for exosomal proteins CD9, CD63, and TSG101. Tubulin in cell extracts was used as a control for cell density. (d) Flow cytometry analyzed the effect of H/N-Exos on the cell cycle of normoxic CRC cells. (e) CCK-8 assay detected the effect of H/N-Exos on the proliferation of normoxic CRC cells. (f) Annexin V-FITC/PI assay detected the effect of H/N-Exos on the apoptosis of normoxic CRC cells. (g–i) The effect of H-Exos depletion on the cell cycle, proliferation, and apoptosis of normoxic CRC cells. Each experiment was carried out at least three times. *P < 0.05; **P < 0.01; and ***P < 0.001. (A color version of this figure is available in the online journal.)