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. 2021 May 8;246(17):1895–1906. doi: 10.1177/15353702211011576

Figure 2.

Figure 2.

miR-210-3p is highly enriched in H-Exos and can be transferred to normoxic CRC cells via exosomes. (a) qRT-PCR analysis of mature miR-210 levels in normoxic HCT116 cells treated with N/H-Exos. (b) qRT-PCR analysis of pri/pre miR-210 levels in normoxic HCT116 cells treated with N/H-Exos. (c) qRT-PCR analysis of the effect of RNase, RNase A+Triton-X, and RNA POLY II inh on miR-210 expression in normoxic HCT116 cells mediated by N/H-Exos. (d) qRT-PCR analysis of mature miR-210 levels in normoxic HCT116 cells treated with exosomes derived from hypoxic or normoxic CRC cells transfected with miR-210 inhibitor. (e) Immunofluorescence staining showing the uptake of H-Exos in normoxic HCT116 cells; Scale bar, 50 μm. Each experiment was carried out at least three times. ***P < 0.001. n.s.: not significant. (A color version of this figure is available in the online journal.)