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Ligustilide restrained nuclear translocation and activity of YAP in HCC cells. (a) The mRNA levels of YAP were detected in HCC cells after ligustilide treatment by qRT-PCR. (b) Then, luciferase activity of 8xGTIIC was applied to measure the transcriptional activity of YAP. (c) The expression of CTGF was determined. (d) The protein levels of YAP in nucleus were analyzed by Western blotting. (e) Total YAP and p-YAP protein levels were also measured. *P < 0.05.
