Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 8;40:284. doi: 10.1186/s13046-021-02088-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — ALKBH5-HOXA10 loop maintains ALKBH5 and HOXA10 overexpression in EOC. (a) The results of the RIP-qPCR assay show that HOXA10 mRNA is enriched in Flag-specific antibody in EOC cells on transfection with oe-ALKBH5 using Flag-tag. (b) The Act-D assay shows that ALKBH5 overexpression can maintain HOXA10 mRNA stability in EOC cells. (c and d) ALKBH5 regulates HOXA10 expression in EOC. (e) The ChIP-qPCR assay suggests that the ALKBH5 promoter fragment is enriched in HOXA10 (top), and the AGE assay validates the sonicated products and immunoprecipitated DNA of the ChIP assay (middle). EOC cells are transfected with HOXA10-Flag-tag (bottom). (f) Based on the TF binding motif of HOXA10 predicted by the JASPAR database (top), the luciferase reporter assay is conducted, and the results show that HOXA10 could interact with the region containing TAAA of ALKBH5 promoter (bottom). (g) The transfection efficiency of oe-HOXA10 lentivirus in A2780 and HO8910. (h) ALKBH5 is up-regulated in EOC cells with HOXA10 overexpression. (i) The transfection efficiency of specific siRNAs targeting HOXA10. (j) ALKBH5 expression is down-regulated by knocking down HOXA10 in EOC cells