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. 2021 Sep 8;40:284. doi: 10.1186/s13046-021-02088-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — HOXA10 overexpression promotes EOC cell proliferation and cisplatin resistance in vitro and in vivo. (a) HOXA10 mRNA is upregulated in cisplatin-resistant EOC cells and platinum-resistant EOC samples. (b) Results of the western-blot assays shows HOXA10 was up-regulated both in cisplatin-resistant EOC cells (upper) and platinum-resistant EOC samples (down). (c) Expression of HOXA10 protein in platinum-resistant (left) and platinum-sensitive (right) EOC tissues by IHC assay. (d and e) CCK8 and EdU proliferation assays confirm that HOXA10 overexpression promotes cell proliferation in EOC cells. (f) The chemosensitivity assay demonstrates higher IC50 of cisplatin in EOC cells with oe-HOXA10 transfection. (g) The IF assay shows that γH2AX foci is decreased in EOC cells with HOXA10 overexpression after cisplatin treatment (5 μM, 6 h). (h) The cell cycle assays show that HOXA10 overexpression can alleviate the blocking in the G2/M phase induced by cisplatin (5 μM 48 h). (i) The apoptosis assays show that HOXA10 overexpression can lower the percentage of cell apoptosis induced by cisplatin (5 μM, 48 h). (j) The animal study shows that HOXA10 overexpression promotes cell proliferation and resistance to cisplatin in vivo