Fig. 3.

Short-chain fatty acid- and secondary bile acid-producing bacteria positively correlate with colonic P-gp expression. A Volcano plot showing the rho coefficient and corresponding p value of Spearman’s correlation test of relative abundance of microbial genes from whole genome sequencing data (regrouped to KO groups) relative to expression of P-gp by western blot densitometry in each mouse. Significance set at p < 0.05. KOs involved in butyrate and secondary bile acid metabolism pathways are labeled. B Positively correlated KOs identified in A with corresponding enzyme commission (EC) numbers, definition, and involved pathways being indicated