Fig. 6.

A) One-pot synthesis of d-cellobiose from sucrose catalysed by sucrose phosphorylase (SCP), glucose isomerase (GI) and cellobiose phosphorylase (CBP). B) Enzymatic platform for the synthesis of various disaccharides from starch employing isoamylase (IA), α-glucosidase (αG) and α-glucan phosphorylase (αGP) in parallel, and a disaccharide phosphorylase such as CBP or laminaribiose phosphorylase (LBP). C) d-laminaribiose synthesis from sucrose and D-Glc in one-pot reaction containing individually immobilised SCP and E. gracilis extract with enriched LBP activity. D) Cascade synthesis of d-laminaribiose from starch and glucose using IA, αGP, LBP and 4-α-glucanotransferase (4GT), which was used to recycle maltose into malto-oligosaccharides for continuous production of α-D-Glc-1P.