Extended Data Fig. 1: The lectin pathway and the C3aR promote 3-MCA-induced sarcomagenesis and FS6 sarcoma growth.

(a-h) 3-MCA-induced sarcoma growth curves (left panels) or mean tumor volume (± SEM, right panels) in C3^−/− (a, n=20 wt, n=18 ko), MBL1/2^−/− (b, n=10 wt, n=14 ko), C4^−/− (c, n=10 mice in each group), C1q^−/− (d, n=7 wt, n=8 ko), fB^−/− (e, n=9 wt, n=12 ko), C5aR1^−/− (f, n=10 wt, n=19 ko), C5aR2^−/− (g, n=14 wt, n=10 ko) and C3aR^−/− (h, n=14 wt, n=16 ko) mice. Experiment of panel d was performed using wt littermates. (i) Representative H&E staining of 3-MCA tumor tissues of wt, C3^−/−, MBL1/2^−/−, C4^−/− and C3aR^−/− mice. One experiment performed, three representative fields have been acquired for each group (n=4 mice in each group). Scale bar: 100μm. (j-q) FS6 tumor incidences in C3^−/− (j, n= 4 wt, n=8 ko); MBL1/2^−/− (k, n=10 wt, n=9 ko); C4^-/ (l, n=11 wt, n=10 ko); C3aR^−/− (m, n=15 wt, n=12 ko); C1q^−/− (n, n=15 wt, n=8 ko); fB^−/− (o, n=9 wt, n=4 ko); C5aR1^−/− (p, n=12 wt, n=5 ko) and C5aR2^−/− (q, n=12 wt, n=8 ko) mice. (r-u) FS6 tumor volumes (mean ± SEM) (r, t) and incidences (s, u) in MBL1/2^±/− (r-s, n=12 he, n=8 ko) and C3aR^±/− (t-u, n=21 he, n=12 ko) littermates. One representative experiment out of three (a), two (b, d, e, f, j and k) or one (c, g, h, l, n, p, q, r, s, t and u) performed is shown. o, m: two pooled experiments. The same wt mice were used simultaneously as control mice of experiments of panels p, q and one out of the two pooled experiments of panel m. Two-tailed Mann Whitney test (r) or unpaired two-tailed Student’s t test (t). Exact p values are reported, two-tailed Wilcoxon matched-pairs signed rank test (j-q, s and u).