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. 2020 Jun 8;4(7):2000062. doi: 10.1002/adbi.202000062

Figure 2.

Figure 2

Cell adhesion of AdMSC on TIPS microcarriers. A) Confocal microscopy images of phalloidin (green) and Hoechst (blue) stained AdMSC attached to the surface of TIPS microcarriers composed of polymer 1 i), polymer 2 ii), and polymer 3 iii) microcarriers after 6 h incubation. Scale bar: 100 µm. B) Quantitative evaluation of cell adhesion on TIPS microcarriers after 6 h. i) relative quantity of TIPS microcarriers with cells attached within a specific range (data from three experiments; n = 70–90 TIPS microcarriers per type and experiment). ii) Surface coverage of microcarriers by cells per single TIPS microcarrier. Coverage is expressed as mean % ± standard deviation (STDEV) (≈120 TIPS microcarriers measured per polymer type). C) SEM images of AdMSC adhered to the surface of a TIPS microcarrier composed of polymer 1 for 24 h i,ii) (scale bar in Ci is 20 µm; scale bar in Cii is 50 µm); iii,iv) brightfield microscopy images of AdMSC attached to a single microcarrier in the hanging drop culture at 48 h postseeding: iii) a solid microcarrier and iv) TIPS microcarrier composed of polymer 3 (scale bar in Ciii and Civ is 100 µm).