Figure 4.

Evaluation of the angiogenic secretome from AdMSC cultured on TIPS microcarriers. A) Human VEGF in the secretome of AdMSC cultured on different compositions of TIPS microcarriers or tissue culture (TC) plastic. The results are presented as i) VEGF concentration (pg mL⁻¹) and ii) adjusted for VEGF per DNA (pg µg⁻¹). The level of significance was calculated using the one‐way ANOVA followed by Tukey test for multiple comparisons between the “TC” condition and the test samples (TIPS microcarriers) (*p < 0.05, **p < 0.01, and ***p < 0.001; the data represent three independent experiments). B) The relative amount of soluble angiogenesis‐related proteins in the pooled supernatants from cellularized TIPS microcarriers and solid microcarriers composed of polymer 3 and PS solid microcarriers using the human proteome profiler angiogenesis array. Heat maps for the analytes were generated by quantifying the mean spot pixel densities from two separate experiments in the array membrane using image software. The data were normalized against the values obtained for the control PS microcarriers.