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. Author manuscript; available in PMC: 2022 Aug 15.
Published in final edited form as: J Physiol. 2021 Jun 23;599(16):3973–3991. doi: 10.1113/JP281698

Figure 6: Aging results in an enhanced proinflammatory phenotype of mesenteric accumulating T cells.

Figure 6:

To assess cytokine production, mesenteric single cell suspensions from young (n = 6) and old (n = 8) mice were activated in vitro. Cells were then stained for CD45 (total leukocytes), CD3 (pan T cells), CD4, CD8, interferon (IFN)-γ and tumor necrosis factor (TNF)-α. (A) Sample IFN-γ flow cytometry plots. (B) Proportion and (C) number of IFN-γ producing T cells. (D) Sample TNF-α flow cytometry plots. (E) Proportion and (F) number of TNF-α producing T cells. Group differences were assessed with an independent samples T test. p values are included on the panel with a horizontal line indicating the group comparison. Data are shown as mean ± standard deviation, n represents the number of independent animals in each group.