Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 25;11:706252. doi: 10.3389/fcimb.2021.706252

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Ren, Wang, Fang, Shu, Wu, Wang, Huang, Min, Jin, Zhou, Qiu and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

SARS-CoV-2 N enhances M-induced apoptosis via interacting with both M and PDK1. (A) Schematic representation of SARS-CoV-2 N and its truncations. (B, C) HEK293T cells transfected with HA-SARS-CoV-2 N and its truncations together with FLAG-SARS-CoV-2 M and GFP-PDK1, respectively. After 24 h, cells were collected for co-immunoprecipitation (with anti-FLAG or anti-GFP) and Western blotting (with anti-GFP, anti-HA, or anti-FLAG). (D) Vero E6 and HepG2 cells were transfected with FLAG-SARS-CoV-2 M together with HA-SARS-CoV-2 N and its truncations, respectively. After 24 h, cells were collected for Western blotting (with anti-FLAG, anti-HA, or anti-β-Actin) or stained with Annexin V-FITC/PI for flow cytometry analysis, and the percentage of apoptotic cells were measured. (E) HEK293T cells transfected with FLAG-SARS-CoV-2 M and its truncations together with HA-N. After 24 h, cells were collected for coimmunoprecipitation (with anti-FLAG) and Western blotting (with anti-FLAG or anti-HA). (F) Vero E6 and HepG2 cells were transfected with FLAG-SARS-CoV-2 M and FLAG-SARS-CoV-2 M TM(1 + 2) together with HA-SARS-CoV-2 N. After 24 h, cells were collected for Western blotting (with anti-FLAG, anti-HA, or anti-β-Actin) or stained with Annexin V-FITC/PI for flow cytometry analysis, and the percentage of apoptotic cells were measured. (G) A model for SARS-CoV-2 N enhancing the interaction of M and PDK1. ***P < 0.001 by two-tailed Student’s t-test. ns, non significant.