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The prediction targets and pathways of network pharmacology were validated by Western blot. (A) The expression of PI3K, p-PI3K, AKT, and p-AKT, mTOR, p-mTOR was detected by Western blot. (B) The significant difference of p-PI3K, p-AKT, and p-mTOR was revealed among the three groups. (C) Expression of top 2 targets including STAT3 and MAPK14 was investigated by Western blot. (D) Statistical analysis of Western blot. *p < 0.05, **p < 0.01, ***p < 0.001.
