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Augmented volumetric imaging using an electrically tunable lens controlled by a square-wave voltage V ETL. (a) Multi-Z images obtained for V ETL = 0 and V ETL = 5 V corresponding to a focal shift of 150 μm. (b) Resulting volumetric acquisition of fixed mouse brain vasculature with structures color-coded by depth. 512 × 512 pixels; scale bar 300 μm.
